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	MRA-210 M1-KF Plasmodium falciparum (Primers and Probes)

Organism:	Plasmodium falciparum
Descriptions:	M1-KF
Depositors:	Malaria Research and Reference Reagent Resource Center
Biosafety Level:	1
Shipped:	freeze-dried
Comments:	Quantity limit per order for this item is 1. This item can be ordered once a year. Orders over this limit will be sent to NIAID for approval before shipment. Dissolving the contents of each vial (2.5 nmol) in 0.1 ml of distilled water with its partner primer (total volume 0.2 ml) will allow for 200 PCR reactions at 1 microliter per reaction when used at 500 nM. Dissolving the contents of each vial (2.5 nmole) in 100µl of distilled water and mixing with its partner primer ( total volume of 200µl), it can be used for 200 PCR reactions (each reaction uses 1 µl in a 25µl volume) at 500 nM. If used at 250 nM, 400 reactions can be run. If used at 250 nM, 400 reactions can be run. If three or four primers are used, the above volumes would be 1.5 and 2.0 microliters, respectively, per reaction. Sequence (5' to 3'): AAA TGA AGA AGA AAT TAC TAC AAA AGG TGC [53405] Purity: Standard %GC: 30 Primer Length: 30 This primer is used for the detection of [merozoite surface protein 1] (Plasmodium falciparum) MR4 comment- variant MSP1 oligo target; matches P. falciparum 3D7 MSP1
Store at:	-20°C
Availability Status:	In Stock
References:	53405: Snounou G, et al. Biased distribution of msp1 and msp2 allelic variants in Plasmodium falciparum populations in Thailand. Trans. R. Soc. Trop. Med. Hyg. 93: 369-374, 1999. PubMed: 10674079
Citations:	Acknowledgment for publications should read "The following reagent was obtained through the MR4 as part of the BEI Resources Repository, NIAID, NIH: M1-KF, MRA-210."

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