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MRA-471  Mus musculus (B cell); Mus musculus (myeloma) N1-1H10
(Cell Banks)
Organism: Mus musculus (B cell); Mus musculus (myeloma)
Designations: N1-1H10
Depositors: CA Long
Biosafety Level: 1
Shipped: frozen
Antisera: Antibody Form: monoclonal
Antigen: Plasmodium vivax Pvs25 protein
Conjugated Immunoglobulin: N
Immunoglobulin Class: IgG
Immunoglobulin Subclass: 1
Purified Immunoglobulin: N
Cell Lines: Cytogenic Report: N
Differentiated Cell: Y
Feeder Layer Cell: N
Hybridoma: Y
Cell Type Descriptor: lymphoblast
Cytopathological State: somatic cell hybrid
Sources: Developmental Stage: adult
Tissue State: normal
Strain: 0
Subculturing: Protocol: Thawing of Hybridomas1. Add 7 ml of Dulbecco's modified Eagles medium (DMEM - e.g., Gibco-BRL Cat#1 1995-065) to a 15 ml tube.2. Quickly thaw one vial of cells in a 37C water bath.3. As soon as the ice melted, transfer the cell suspension into the tube with 7 ml DMEM and mix well.4. Spin the cells at 200 rpm in a clinical centrifuge for 3 mins.5. Aspirate the supematant.6. Resuspend the cells in 1 ml of DMEM with 10% fetal bovine serum (DMEN-10%FBS) and transfer it to ONE well of 6-well plate.7. Add an additional 2 ml of DMEM-10%FBS.8. Put the plate into a C02 incubator at 37C.9. 24 hrs later, examine the cells under microscope.10. If there are too many floating cells and/or dead cells, transfer the culture supernatant to another well of 6-well plate and add 3 ml of fresh DMEM-10% FBS to the original well.11. Incubate the cells in a C02 incubator at 37C.12. When the cells reach 75% confluence, split the cells 1:3 ? 1:5 in DMEM-10% FBS.Note:1. These hybridomas may attach to the dish pretty tight. For subculturing, you can mechanically wash up the cells by strong pipeting.2. These hybridomas do not like high cell density and viability drops significantly once over-confluence.
Subcultivation Ratio: 1:3 to 1:5
Medium Renewal: every 2 to three days
Tissue: spleen; B lymphocyte
Isotype: IgG1
Store at:   LN2
Applications: malaria transmission studies
vaccine studies
Comments: Quantity limit per order for this item is 1. This item can be ordered once a year. Orders over this limit will be sent to NIAID for approval before shipment.


Cells were tested for Mycoplasma contamination and results were negative.
Availability Status: In Stock
Citations: Acknowledgment for publications should read "The following reagent was obtained through the MR4 as part of the BEI Resources Repository, NIAID, NIH: Mus musculus (B cell); Mus musculus (myeloma) N1-1H10, MRA-471, deposited by CA Long."
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