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MRA-53  yP30P2 PfMSP1-19(Q-KNG)FVO/VK1 Plasmodium falciparum
(Proteins)
Organism: Plasmodium falciparum
Descriptions: yP30P2 PfMSP1-19(Q-KNG)FVO/VK1
Depositors: DC Kaslow
Biosafety Level: 1
Shipped: frozen
Comments: Quantity limit per order for this item is 1. This item can be ordered once a year. Orders over this limit will be sent to NIAID for approval before shipment.

Description: Yeast-secreted 19 kDa recombinant protein containing the carboxy terminus of MSP1 from P. falciparum fused to P30P2.
Protein size: 19 kDa
Protein function: antigen
Tissue/cellular location: merozoite surface
Gene coding for protein: P30P2 MSP1-19
Purification methods: microfiltration, tangential utrafiltration diafiltration, NI-NTA purification, size-exclusion chromatography (Superdex 75)
Quality control methods: SDS-PAGE
Additional information: The protein is secreted and recoverable from the culture supernatant.
The protein is expressed in Saccharomyces cerevisiae strain VK1 as a recombinant protein using the pIXY154 expression plasmid.
The MSP1-19 fragment consists of Asn1631 to Ser1723 as a PCR amplified product from the FVO strain with a His6 tag.
Store at: -70°C
Availability Status: In Stock
References: 39877: . Immunogenicity and in vivo efficacy of recombinant Plasmodium falciparum merozoite surface protein-1 in Aotus monkeys. Mol. Med. 1: 325-32, 2000. PubMed: 8529111
Citations: Acknowledgment for publications should read "The following reagent was obtained through the MR4 as part of the BEI Resources Repository, NIAID, NIH: Plasmodium falciparum yP30P2 PfMSP1-19(Q-KNG)FVO/VK1, MRA-53, deposited by DC Kaslow."
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