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	NR-10435 Bacillus anthracis Spore Coat Protein GerQ (Locus Tag: BA_5641) with N-terminal Histidine Tag, Recombinant from Escherichia coli (Proteins)

Description:	Spore Coat Protein GerQ (Locus Tag: BA_5641) with N-terminal Histidine Tag, Recombinant from Escherichia coli
Organism:	Bacillus anthracis
Biosafety Level:	1
Availability Status:	In Stock
Store at:	-80°C or colder
Contributor:	AD O'Brien, JF Sinclair
Comments:	Quantity limit per order for this item is 3. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. NR-10435 is a recombinant form of the Bacillus anthracis (B. anthracis) spore coat protein GerQ (also YwdL; locus_tag: BA_5641), a component of the proteinaceous layer surrounding the spore's outer membrane. The amino acid sequence includes 1) an N-terminal hexa-histidine tag, 2) a thrombin cleavage site and 3) the complete coding sequence (amino acid residues 1 to 145) of GerQ from the Ames strain (GenPept: AAP29276). The recombinant protein was expressed in Escherichia coli and purified by nickel affinity chromatography. NR-10435 has a theoretical molecular weight of approximately 18.5 kilodaltons. NR-10435 reacts with rabbit polyclonal antibody to B. anthracis GerQ (BEI Resources NR-10436) as shown by Western blot analysis. Each vial contains approximately 10 µg of NR-10435 in PBS.
Citations:	Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Bacillus anthracis Spore Coat Protein GerQ (Locus Tag: BA_5641) with N-terminal Histidine Tag, Recombinant from Escherichia coli, NR-10435."

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