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	NR-13311 Plasmid pMRLB.61 Containing Gene Rv0432 (Protein SodC) from Mycobacterium tuberculosis for Expression in Mycobacterium smegmatis (Plasmid/Vectors)

Description:	Plasmid pMRLB.61 Containing Gene Rv0432 (Protein SodC) from Mycobacterium tuberculosis for Expression in Mycobacterium smegmatis
Organism:	Mycobacterium tuberculosis
Biosafety Level:	1
Availability Status:	In Stock
Store at:	-20°C or colder
Contributor:	NIH - TB Vaccine Testing and Research Materials Contract
Comments:	Quantity limit for this item is 2 per year. Orders over this limit will be sent to NIAID for approval before shipment. NR-13311 is a recombinant expression vector containing Mycobacterium tuberculosis gene Rv0432, which encodes a probable periplasmic superoxide dismutase, SodC. Gene Rv0432 was amplified by PCR and cloned into pVV16 for expression in Mycobacterium smegmatis. The gene was cloned with a signal sequence. The expressed protein is histidine-tagged and has an observed molecular weight of 24 kDa. Note: Plasmid pMRLB.61 contains the gene required for kanamycin (kan) resistance. The recommended concentration of kan in culture is 25 µg/mL. Each vial contains 1 µg of plasmid DNA in 10 mM Tris-HCl, pH 7.5.
Citations:	Acknowledgment for publications should read "The following reagent was obtained through the NIH Biodefense and Emerging Infection Research Resources Repository, NIAID, NIH: Plasmid pMRLB.61 Containing Gene Rv0432 (Protein SodC) from Mycobacterium tuberculosis for Expression in Mycobacterium smegmatis, NR-13311."

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