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	NR-13360 Genomic DNA from Francisella tularensis subsp. novicida, Strain ΔIglC (Nucleic Acids)

Description:	Genomic DNA from Francisella tularensis subsp. novicida, Strain ΔIglC
Organism:	Francisella tularensis subsp. novicida
Biosafety Level:	1
Availability Status:	In Stock
Store at:	-20°C or colder
Contributor:	FE Nano
Comments:	Quantity limit per order for this item is 1. This item can be ordered once a year. Orders over this limit will be sent to NIAID for approval before shipment. Genomic DNA was isolated from a preparation of Francisella tularensis subsp. novicida, strain ΔIglC. Francisella tularensis subsp. novicida, strain ΔIglC is a transposon mutant of the wild-type strain U112, in which the iglC gene region has been replaced with a mini-Tn5 insert, rendering it resistant to kanamycin. The subspecies designation of strain ΔIglC has been confirmed by PCR amplification of an approximately 3300 base pair subspecies specific sequence (RD-1; Region of Difference-1) from extracted DNA. NR-13360 has been qualified for PCR applications by amplification of approximately 1500 bp of the 16S ribosomal RNA gene. Each vial contains 4 to 6 µg of bacterial genomic DNA in TE buffer (10 mM Tris-HCl and 1 mM EDTA, pH ~ 8.0).
Citations:	Acknowledgment for publications should read "The following reagent was obtained through the NIH Biodefense and Emerging Infections Research Resources Repository, NIAID, NIH: Genomic DNA from Francisella tularensis subsp. novicida, Strain ΔIglC, NR-13360."

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