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	NR-14871 Mycobacterium tuberculosis Ag85A Recombinant Protein Reference Standard (Proteins)

Description:	Ag85A Recombinant Protein Reference Standard
Organism:	Mycobacterium tuberculosis
Biosafety Level:	1
Availability Status:	In Stock
Store at:	-80°C or colder
Contributor:	NIH - TB Vaccine Testing and Research Materials Contract
Comments:	Quantity limit for this item is 1 per year. Orders over this limit will be sent to NIAID for approval before shipment. NR-14871 is a recombinant form of the antigen 85 complex A (Ag85A) protein. The recombinant protein consists of the native protein sequence in addition to a hexa-histidine tag. The recombinant protein was expressed in Escherichia coli and purified using standard chromatographic techniques followed by endotoxin removal procedures. Ag85A is one of three components (Ag85A, Ag85B, Ag85C) of the secreted immunodominant 30-32 kDa Antigen 85 Complex present in the culture filtrate of Mycobacterium tuberculosis (M. tuberculosis). Each of the three proteins are involved in cell wall formation and have been linked to disease pathogenesis through their fibronectin-binding abilities. Antigen Ag85A induces strong T-cell proliferation and interferon-γ in individuals infected with M. tuberculosis. Note: This protein is provided as a reference standard and should be ordered with the corresponding plasmid (pMRLB.41; NR-13292). Each vial contains approximately 1 mg of lyophilized NR-14871 in 10 mM ammonium bicarbonate. Some lots of NR-14871 may have been vialed by ATCC ^® rather than by Colorado State University under the TB Vaccine Testing and Research Materials Contract. At ATCC ^® the material provided by Colorado State University was rehydrated, aliquoted into vials and lyophilized.
Citations:	Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Ag85A Recombinant Protein Reference Standard, NR-14871."

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