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NR-15475   Monoclonal Anti-Epsilon Toxin from Clostridium perfringens, Clone P5C10C5 (produced in vitro)
(Monoclonal Antibodies)
Description: Monoclonal Anti-Epsilon Toxin from Clostridium perfringens, Clone P5C10C5 (produced in vitro)
Organism: Clostridium perfringens
Biosafety Level: 1
Availability Status: In Stock
Store at: -20°C or colder
Contributor: BEI Resources
Comments:
Quantity limit per order for this item is 3. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

NR-15475 is being provided without confirmation of functional activity. However, culture supernatant from hybridoma clone P5C10C5 was shown to react with recombinant Clostridium perfringens (C. perfringens) epsilon toxin by western blot and ELISA.

Hybridoma clone P5C10C5 produces IgG2b and IgM. NR-15475 was purified from clone P5C10C5 supernatant and contains both IgG2b and IgM. Please read the Certificate of Analysis carefully to determine whether this product is acceptable for your intended use.

Antibody Class: IgG2bκ and IgMκ

Monoclonal antibody prepared against recombinant epsilon toxin from C. perfringens was purified from hybridoma clone P5C10C5 supernatant by protein G affinity chromatography. The B cell hybridoma was generated by the fusion of Sp2/0-Ag14 myeloma cells with splenocytes from BALB/c mice immunized with purified recombinant protein.

Each vial of NR-15475 contains 40 µg to 60 µg of purified antibody in PBS.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Monoclonal Anti-Epsilon Toxin from Clostridium perfringens, Clone P5C10C5 (produced in vitro), NR-15475."
ATTACHMENTS
Certificate of Analysis
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