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	NR-15491 Vaccinia Virus, Western Reserve, Recombinant Expressing Junin Virus, XJ13 L Protein (Viruses)

Description:	Vaccinia Virus, Western Reserve, Recombinant Expressing Junin Virus, XJ13 L Protein
Organism:	Junin virus
Biosafety Level:	2
Availability Status:	In Stock
Store at:	-60°C or colder
Contributor:	MJ Buchmeier
Comments:	Quantity limit per order for this item is 1. This item can be ordered once a year. Orders over this limit will be sent to NIAID for approval before shipment. A cDNA clone containing the entire ORF encoding the L (polymerase) protein from segment L of Junin virus (JUNV), XJ13 was inserted into the pRB21 transfer vector, bringing it under the control of a synthetic vaccinia virus (VACV) early/late promoter (PSYN). Recombinant VACV was made by transfecting the transfer plasmid into CV-1 cells infected with the VACV strain vRB12. JUNV is an Arenavirus (Arenaviridae, Arenavirus) which is the etiologic agent of Argentine hemorrhagic fever. Each vial contains approximately 1 mL of cell lysate and supernatant from Cercopithecus aethiops kidney epithelial cells (BSC-40, ATCC ^® CRL-2761™) infected with vaccinia virus, rVACV-JUNV XJ13 L. Note: If homogeneity is required for your intended use, please purify prior to initiating work.
Citations:	Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Vaccinia Virus, Western Reserve, Recombinant Expressing Junin Virus, XJ13 L Protein, NR-15491."

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