To access your BEI Web Portal Account:

Login 
    OR
Choose a Collection
 OR
Choose an Organism
Back to My Search

Login is required to view Product Sheets and Certificates of Analysis.
Contact Us   Having technical issues or questions regarding this product?
Please Contact Us.
BEI Resources currently limits the total number of "Made to Order" items which can be ordered by a registrant to 10 "Made to Order" items per 6 month period. Please check the Availability Status on the items you are ordering and limit orders appropriately.
NR-19274   Mycobacterium tuberculosis Gateway® Clone Set, Recombinant in Escherichia coli, Plates 1-42
(Clones)
Description: Mycobacterium tuberculosis Gateway® Clone Set, Recombinant in Escherichia coli, Plates 1-42
Organism: Mycobacterium tuberculosis
Biosafety Level: 1
Availability Status: In Stock
Store at: -80°C or colder
Contributor: Pathogen Functional Genomics Resource Center at the J. Craig Venter Institute
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

NR-19274 consists of Plates 1-42 (BEI Resources NR-19637 through NR-19678) of the Mycobacterium tuberculosis (M. tuberculosis) Gateway® clone set. Detailed information is available on the Product Information Sheet and Certificate of Analysis for each individual plate.

The M. tuberculosis Gateway® clone set (Plates 1-42) contains 3724 sequence validated clones (3294 M. tuberculosis, strain H37Rv clones supplemented with 430 unique open reading frames (ORF) from M. tuberculosis, strain CDC1551) cloned in Escherichia coli (E. coli) DH10B-T1 cells. Each open reading frame was constructed in vector pDONR 221 with an ATG start codon and no stop codon. The sequence was validated by full length sequencing of each clone with greater than 1X coverage and a mutation rate of less than 0.2%.

Information related to the use of Gateway® Clones can be obtained from Invitrogen™. Recombination was facilitated through an attB substrate (attB-PCR product or a linearized attB expression clone) with an attP substrate (pDONR™ 221) to create an attL-containing entry clone. The entry clone contains recombinational cloning sites, attL1 and attL2 to facilitate gene transfer into a destination vector, M13 forward and reverse priming sites for sequencing and a kanamycin resistance gene for selection. Please refer to the Invitrogen™ Gateway® Technology Manual for additional details.

Every inoculated well of each 96-well plate contains approximately 40 µL of E. coli culture (strain DH10B-T1) in Luria Bertani (LB) Broth containing 50 µg/mL kanamycin supplemented with 15% glycerol.

Note: Production in the 96-well format has a potential for cross-contamination. Individual mutants should be checked by the recipient prior to use.

Additional information is available at the Pathogen Functional Genomics Resource Center at the J. Craig Venter Institute.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Mycobacterium tuberculosis Gateway® Clone Set, Recombinant in Escherichia coli, Plates 1-42, NR-19274."
ATTACHMENTS
PERMITS
Select the country to see the list of permits that may be required for shipping this product.

Return to Top


Notices and Disclaimers

BEI Resources products are intended for laboratory research purposes only. They are not intended for use in humans.

While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this site, BEI Resources makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. BEI Resources does not warrant that such information has been confirmed to be accurate.


Back to My Search