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	NR-19333 SDS-Soluble Cell Wall Fraction (MLCwA) Mycobacterium leprae (Antigen Preparations)

Description:	SDS-Soluble Cell Wall Fraction (MLCwA)
Organism:	Mycobacterium leprae
Biosafety Level:	1
Availability Status:	In Stock
Store at:	-80°C or colder
Contributor:	NIH - Leprosy Research Support Contract
Comments:	Quantity limit per order for this item is 2. This item can be ordered three times per year. Orders over this limit will be sent to NIAID for approval before shipment. NR-19333 was produced from purified Mycobacterium leprae (M. leprae) whole cells (non-irradiated, non-autoclaved) that were sonicated for forty, one minute cycles. An acid fast stain of the sonicate was performed to confirm complete breakage of the cells. The sonicate was centrifuged to pellet the cell wall material which was washed three times with phosphate buffered saline to remove any contaminating cytosolic or membrane proteins. The washed pellet was resuspended in phosphate buffered saline and 2% sodium dodecyl sulfate (SDS) and heated. After one hour the mixture was centrifuged to remove the insoluble material. After two additional extractions the extracts were pooled and passed over an Extractigel-D column to remove the SDS. The final preparation was quantitated for protein content by BCA assay. Each vial contains approximately 0.5 mg of lyophilized NR-19333. NR-19333 can be reconstituted in sterile phosphate buffered saline, pH 7.2, or another suitable buffer.
Citations:	Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Mycobacterium leprae SDS-Soluble Cell Wall Fraction (MLCwA), NR-19333."

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