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NR-21973  Human respiratory syncytial virus Human Reference Immune Globulin to Respiratory Syncytial Virus, CBER RSV Lot 1
(Polyclonal Antiserum)
Description: Human Reference Immune Globulin to Respiratory Syncytial Virus, CBER RSV Lot 1
Organism: Human respiratory syncytial virus
Biosafety Level: 1
Availability Status: In Stock
Store at: -20°C or colder
Contributor: U.S. Food and Drug Administration
Comments:
Quantity limit for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Human plasma was obtained from normal healthy donors, some of whom had probably been infected with respiratory syncytial virus (RSV) in the past. The immune globulin was purified from the plasma pools using a modified Cohn-Oncley cold ethanol fractionation process, as well as cation and anion exchange chromatography. The immune globulin was further treated to remove or inactivate viruses using solvent detergent treatment (with tri-n-butyl phosphate, octoxynol 9, and polysorbate 80 at 18°C to 25°C for a minimum of 60 minutes), 35 nm filtration and low pH incubation at an elevated temperature. The final immune globulin (10%) is a sterile 100 mg/mL protein preparation stabilized with 0.25 M glycine (pH 4.6 to 5.1). At least 98% of the protein was determined to be immune globulin. The osmolality was determined to be 240 to 300 mOsmol/kg.

Each vial contains approximately 2 mL of immune globulin (10%) in 0.25 M glycine (pH 4.6 to 5.1).

Note: Dilute 1:10 to a final concentration of 1% immune globulin in sterile PBS (pH 7.4) prior to testing in virus neutralization assays.

NR-21973 is also available as part of a Panel of Human Antiserum and Immune Globulin to Respiratory Syncytial Virus (BEI Resources NR-32832).
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Human Reference Immune Globulin to Respiratory Syncytial Virus, CBER RSV Lot 1, NR-21973."
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