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NR-2624  Vaccinia virus (WR) B5R Protein with N-terminal Histidine Tag, Recombinant from Baculovirus
(Proteins)
Description: (WR) B5R Protein with N-terminal Histidine Tag, Recombinant from Baculovirus
Organism: Vaccinia virus
Biosafety Level: 1
Availability Status: Made to Order - Contact Customer Service for Availability Date.
Store at: -20°C or colder
Contributor: GH Cohen, RJ Eisenberg
Comments:
NR-2624 is no longer available. Vaccinia virus (WR) B5R Protein with N-Terminal Histidine Tag, Recombinant from Baculovirus is now available as BEI Resources NR-22132. NR-22132 was expressed from the same recombinant baculovirus vector as NR-2624, but was produced in High-5 insect cells instead of cabbage looper (Trichoplusia ni) insect larvae.

NR-2624 is a recombinant form of the B5R membrane glycoprotein [B5R(275t); residues 20 to 275 comprising the ectodomain, N-terminal histidine-tagged] of the Western Reserve (WR) strain of vaccinia virus. The full length B5R protein is 317 residues (GenPept Q01227). NR-2624 was produced in cabbage looper (Trichoplusia ni) insect larvae using a baculovirus expression vector system and was purified using nickel affinity chromatography. NR-2624 was demonstrated to be functionally active based on its reactivity with human polyclonal anti-vaccinia virus immune globulin (VIG; BEI Resources NR-650) and mouse monoclonal antibodies to B5R (BEI Resources NR-422 to NR-424, NR-426 to NR-431, NR-551 to NR-556 and NR-559 to NR-562).

Each vial contains approximately 1.0 mg of NR-2624 in 25 mM phosphate buffer (pH 7.0) containing 150 mM NaCl/50% glycerol (v/v).
Citations: Acknowledgment for publications should read "The following reagent was obtained through the NIH Biodefense and Emerging Infections Research Resources Repository, NIAID, NIH: Vaccinia Virus (WR) B5R Protein with N-terminal Histidine Tag, Recombinant from Baculovirus, NR-2624."
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