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	NR-3028 Genomic DNA from Francisella tularensis subsp. novicida, Strain KM14S (Nucleic Acids)

Description:	Genomic DNA from Francisella tularensis subsp. novicida, Strain KM14S
Organism:	Francisella tularensis subsp. novicida
Biosafety Level:	1
Availability Status:	In Stock
Store at:	-20°C (or colder)
Contributor:	FE Nano
Comments:	Quantity Limit per Order for this item is 1. This item can be ordered once a year. Orders over this limit will be sent to NIAID for approval before shipment. Genomic DNA was isolated from a preparation of Francisella tularensis subsp. novicida, strain KM14S. F. tularensis subsp. novicida, is a Gram-negative, facultative bacterium, which grows predominantly in macrophages when living in mammalian hosts. It is commonly used for studying F. tularensis pathogenesis since it is highly virulent in mice but has minor effects on humans. F. tularensis subsp. novicida, strain KM14S is a derivative of KM14 strain. The KM14S strain is unable to grow in macrophages and no longer expresses kanamycin resistance. KM14 is a derivative of the wild-type strain U112. NR-3028 has been confirmed as non-type B by PCR amplification of an approximately 390 bp amplicon. Analysis of the 16S sequence indicates that NR-3028 is consistent with other strains of F. tularensis subsp. novicida. NR-3028 has been qualified for PCR applications by amplification of approximately 1500 bp of the 16S ribosomal RNA gene. Each vial contains approximately 4-6 µg of bacterial genomic DNA in TE buffer (10 mM Tris-HCl and 1 mM EDTA, pH 8.0). The vial should be centrifuged prior to opening.
Citations:	Acknowledgment for publications should read "The following reagent was obtained through the NIH Biodefense and Emerging Infections Research Resources Repository, NIAID, NIH: Genomic DNA from Francisella tularensis subsp. novicida, Strain KM14S, NR-3028."

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