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	NR-3049 Genomic DNA from Escherichia coli, Strain B2F1 (Nucleic Acids)

Description:	Genomic DNA from Escherichia coli, Strain B2F1
Organism:	Escherichia coli
Biosafety Level:	1
Availability Status:	In Stock
Store at:	-20°C or colder
Contributor:	ATCC^®
Comments:	Quantity limit per order for this item is 1. This item can be ordered once a year. Orders over this limit will be sent to NIAID for approval before shipment. Genomic DNA was isolated from a preparation of Escherichia coli, strain B2F1, serotype O91:H21. Enterohemorrhagic E. coli (EHEC), strain B2F1 was isolated from a human patient with hemolytic uremic syndrome in Toronto, Canada. E. coli, B2F1 is reported to produce two Shiga-like type II toxins, contain a large hemolysin-encoding plasmid, and is referred to as an EHEC and Shiga toxin-producing E. coli (STEC) strain. Many EHEC strains encode potent toxins, similar to those of Shigella dysenteriae, which can cause severe intestinal, kidney and central nervous system disease. NR-3049 has been qualified for PCR applications by amplification of approximately 1500 bp of the 16S ribosomal RNA gene. The presence of plasmid pO157 has been confirmed by PCR amplification of an approximately 3200 bp sequence. Each vial contains 4 to 6 µg of bacterial genomic DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH ~ 7.4).
Citations:	Acknowledgment for publications should read "The following reagent was obtained through the NIH Biodefense and Emerging Infections Research Resources Repository, NIAID, NIH: Genomic DNA from Escherichia coli, Strain B2F1, NR-3049."

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