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	NR-4745 Monoclonal Anti-Dengue Virus Type 1 Envelope Protein, Clone E17 (produced in vitro) (Monoclonal Antibodies)

Description:	Monoclonal Anti-Dengue Virus Type 1 Envelope Protein, Clone E17 (produced in vitro)
Organism:	Dengue virus type 1
Biosafety Level:	1
Availability Status:	In Stock
Store at:	-20°C or colder
Contributor:	MS Diamond
Comments:	Quantity limit per order for this item is 5. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. Antibody Class: IgG1κ Mouse monoclonal antibody prepared against the envelope glycoprotein of dengue virus type 1 (DEN-1) was purified from clone E17 hybridoma supernatant by protein G affinity chromatography. The B cell hybridoma was generated by the fusion of P3X63.Ag.8.6.5.3 BALB/c mouse myeloma cells with immunized mouse splenocytes. The clone E17 antibody is reported to bind to domain I-II in the envelope glycoprotein. Each vial of NR-4745 contains approximately 100 µL of purified monoclonal antibody in PBS. NR-4745 is reactive in immunofluorescence assays using Vero cells infected with DEN-1 and by ELISA using DEN-1-infected cell lysates (DEN-1, Hawaii; BEI Resources NR-82). The antibody is reported to have neutralizing activity and to be reactive using flow cytometry.
Citations:	Acknowledgment for publications should read "The following reagent was obtained through the NIH Biodefense and Emerging Infections Research Resources Repository, NIAID, NIH: Monoclonal Anti-Dengue Virus Type 1 Envelope Protein, Clone E17 (produced in vitro), NR-4745."

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