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	NR-658 Influenza A virus H5 HA Protein from Influenza Virus, A/Hong Kong/213/2003 (H5N1), Recombinant from Baculovirus (Proteins)

Description:	H5 HA Protein from Influenza Virus, A/Hong Kong/213/2003 (H5N1), Recombinant from Baculovirus
Organism:	Influenza A virus
Biosafety Level:	1
Availability Status:	In Stock
Store at:	4°C
Contributor:	NIH - Influenza Pandemic Preparedness in Asia Program
Comments:	Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. Recombinant H5 hemagglutinin (HA) protein from influenza virus A/Hong Kong/213/2003 (H5N1) was produced in Sf9 insect cells using a baculovirus expression vector system. Recombinant H5 HA protein was purified using conventional chromatographic techniques. NR-658 is weakly active in a hemagglutination assay with 0.5% chicken red blood cells. Prior to vialing, the bulk form of NR-658 possessed a sufficient hemagglutination titer to allow antigenic specificity testing using a serological hemagglutination inhibition (HI) assay. The HI results showed that the bulk H5 HA protein does not react with reference antisera to any of the other HA subtypes. Post-vialing ELISA experiments demonstrated that NR-658 has reactivity with reference antisera within the H5 HA subtype. Analysis by SDS-PAGE indicates that the purified recombinant H5 HA protein preparation may be unstable over time. Each vial contains approximately 0.25 mL (16.5 micrograms) of purified recombinant H5 HA protein in 10 mM sodium phosphate (pH 7.2), 150 mM sodium chloride, and 0.01% Tween-20.
Citations:	Acknowledgment for publications should read "The following reagent was obtained through the NIH Biodefense and Emerging Infections Research Resources Repository, NIAID, NIH: H5 Hemagglutinin (HA) Protein from Influenza Virus, A/Hong Kong/213/2003 (H5N1), Recombinant from Baculovirus, NR-658."

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