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	NR-9901 Mus musculus Microglial Cell Line Derived from TLR4 Knockout Mice (Cell Banks)

Description:	Microglial Cell Line Derived from TLR4 Knockout Mice
Organism:	Mus musculus
Biosafety Level:	2
Availability Status:	In Stock
Store at:	-100°C or colder, preferably in the vapor phase of a liquid nitrogen freezer
Contributor:	D Golenbock
Comments:	Quantity limit per order for this item is 5. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. The murine microglial cell line, NR-9901, was derived using brain tissue from TLR4 (toll-like receptor 4) knockout mice in a C56BI6/J background. The microglial cells were immortalized by infection with the ecotropic transforming replication-deficient retrovirus J2 using techniques described in the literature. Characterization based on immunofluorescence, stimulation assays and flow cytometry demonstrated that the immortalized cell line retains its microglial-specific morphological, functional and surface expression properties. Each vial contains approximately 1 mL of cell culture suspension frozen in cell growth medium (90%) and DMSO (10%) cryo-preservatant. Sufficient cells are provided to initiate at least one new culture.
Citations:	Acknowledgment for publications should read "The following reagent was obtained through the NIH Biodefense and Emerging Infections Research Resources Repository, NIAID, NIH: Microglial Cell Line Derived from TLR4 Knockout Mice, NR-9901."

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