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NR-19439   Enterotoxigenic Escherichia coli Expression Clone Set, Recombinant in Escherichia coli, Plates 1-14
(Clones)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Enterotoxigenic Escherichia coli Expression Clone Set, Recombinant in Escherichia coli, Plates 1-14
Organism: Escherichia coli
Biosafety Level: 2
Availability Status: In Stock
Store at: -80°C or colder
Contributor: Pathogen Functional Genomics Resource Center at the J. Craig Venter Institute
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

NR-19439 consists of Plates 1-14 (BEI Resources NR-19790-NR-19803) of the Entertoxigenic Escherichia coli (E. coli) expression clone set. Information for each plate is available on the Master Clone List.

The Enterotoxigenic Escherichia coli (ETEC) expression clone set consists of 14 plates which contain approximately 917 sequence validated clones from E. coli strains H10407, E24377A and B7A cloned in E. coli DH10B-T1 cells. Each open reading frame was constructed in vector pMCSG7 (a pET21 derivative; for routine HTP purification). The sequence was validated by full length sequencing of each clone with greater than 1X coverage and a mutation rate of less than 0.2%.

Plate orientation and viability were confirmed for each plate of the set.

Every inoculated well of each 96-well plate contains approximately 60 µL of E. coli culture (strain DH10B-T1) in Luria Bertani (LB) broth containing 100 µg/mL ampicillin supplemented with 15% glycerol.

Clone plates are replicated using a BioMek® FX robot. Production in the 96-well format has increased risk of cross-contamination between adjacent wells. Individual clones should be purified (e.g. single colony isolation and purification using good microbiological practices) and sequence-verified prior to use. BEI Resources only confirms the clone plate orientation and viability of randomly picked clones. BEI Resources does not confirm or validate individual clone identities provided by the contributor.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Enterotoxigenic Escherichia coli Expression Clone Set, Recombinant in Escherichia coli, Plates 1-14, NR-19439."
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