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Antibodies/Antisera
BEI Number: NR-2731   
Description: Monoclonal Anti-Influenza Virus H5 Hemagglutinin (HA) Protein (VN04-8), A/Vietnam/1203/04 (H5N1), (ascites, Mouse) Contributor: NIH Biodefense and Emerging Infections Research Resources Repository
Agent: Influenza A virus
Biosafety Level: 1
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Store at: -20°C to -40°C
Citations: Acknowledgment for publications should read "The following reagent was obtained through the NIH Biodefense and Emerging Infections Research Resources Repository, NIAID, NIH: Monoclonal Anti-Influenza Virus H5 Hemagglutinin (HA) Protein (VN04-8), A/Vietnam/1203/04 (H5N1), (ascites, Mouse), NR-2731."
Comments: Quantity limit per order for this item is 5. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Antibody Class: IgG1κ

Mouse monoclonal antibody specific to a recombinant form of the H5 hemagglutinin (HA) protein (GenPept: AAT73274) of the A/Vietnam/1203/04 (H5N1) strain of influenza virus was produced in mouse ascites. Ascites formation was induced by injecting cultured hybridoma cells into the peritoneal cavity of Balb/c mice that had been primed with Incomplete Freund's adjuvant. Antibody-rich ascites fluid was aseptically harvested 1 to 2 weeks following hybridoma cell injection. The harvested ascites fluid was pooled and then clarified using centrifugation and filtration. Sodium azide (0.02%) and gentamycin (0.01%) were added to the pooled ascites fluid prior to vialing and lyophilization. NR-2731 is specific for the H5 HA subtype of the influenza A virus as determined in standard hemagglutination inhibition (HI) assays. NR-2731 also demonstrates high reactivity within the H5 HA subtype of recent Asian isolates of influenza A virus based on HI assays.

Each vial of NR-2731 contains lyophilized (0.2 mL containing 0.02% sodium azide and 0.01% gentamycin) mouse ascites fluid.

NR-2731 should be reconstituted with 0.2 mL of sterile distilled water.
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