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Toxins
BEI Number: NR-857   
Description: Shiga Toxin Type 1, Recombinant from Escherichia coli Contributor: AD O'Brien, JF Sinclair
Source Designation: Escherichia coli    
Biosafety Level: 2    
Permits/Forms: In addition to the BEI Resources MTA, other ATCC and/or regulatory permits may be required for the transfer of this material. Anyone receiving BEI Resources material is ultimately responsible for obtaining the permits. Please click the View Permits button for information regarding the specific requirements for shipment of this item to your location.



Citations: Acknowledgment for publications should read "The following reagent was obtained through the NIH Biodefense and Emerging Infections Research Resources Repository, NIAID, NIH: Shiga Toxin Type 1, Recombinant from Escherichia coli, NR-857."
Comments: Quantity limit per order for this item is 5. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Recombinant Shiga toxin type 1 was expressed in Escherichia coli (E. coli) and purified by affinity chromatography.

The sequences of the structural genes for Shiga toxin from Shigella dysenteriae and Shiga-like toxin type 1 (Stx 1) from E. coli have been determined. The crystal structure of Shiga toxin from Shigella dysenteriae has been solved (PMB: 1DM0)

Each vial of NR-857, lot 6176493 contains approximately 10 µg of Shiga toxin type 1 suspended in 10 mM sodium phosphate buffer (pH 5.7) and 50 mM sodium chloride.

Each vial of NR-857, lot 58338341 contains approximately 14 µg of Shiga toxin type 1 suspended in PBS, pH 7.4.

NR-857 reacts with rabbit polyclonal antibody to Stx 1 and demonstrates cytotoxicity in Vero cells.

For information about toxin amounts excluded from CDC Select Agent regulations, click here.
Store at: -20°C or colder
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