TARGET Research Results
Details
DescriptionPROBABLE TRANSMEMBRANE ALKANE 1-MONOOXYGENASE ALKB (ALKANE 1-HYDROXYLASE) (LAURIC ACID OMEGA-HYDROXYLASE) (OMEGA-HYDROXYLASE) (FATTY ACID OMEGA-HYDROXYLASE) (ALKANE HYDROXYLASE-RUBREDOXIN)
Accession numberRv3252c
Gene namealkB
Gene length1251
Protein length416
Coordinates3630738..3631988
Orientation-
Molecular mass47014.16
Isoelectric point9.35

Database Crossreferences
Deletion Mutant Phenotype
in-vivoguinea pig
 mouse
 rabbit

in-vitroHBMEC
 macrophage

Mutant Survival in DeADMAn (Lamichhane et al., 2005)*

Strain NamePhenotype (Mouse)
JHU3252c-234[no data]
JHU3252c-322[no data]
JHU3252c-999[no data]
JHU3252c-468[no data]
JHU3252c-207Not-Attenuated
JHU3252c-748[no data]
JHU3252c-1001[no data]
JHU3252c-233[no data]
JHU3252c-562[no data]
 

CDC1551 Homolog
ORFMT3350
Essentiality Prediction
Sassetti et al, 2003*non-essential

JHU Transposon Mutant(s) Available
NameMT#Rv#POIGene LengthAvailable from BEI
JHU3252c-322MT3350Rv3252c3221251no (will be available later)
JHU3252c-1001MT3350Rv3252c10011251no (will be available later)
JHU3252c-233MT3350Rv3252c2331251yes
JHU3252c-562MT3350Rv3252c5621251yes
  
For a list of currently available mutants, please visit BEI's transposon mutant webpage.

If we have not disrupted your gene using random mutagenesis, we may be able to create a knockout mutant for you.

References
Lamichhane, G., M. Zignol, N. J. Blades, D. E. Geiman, A. Dougherty, J. Grosset, K. W. Broman, and W. R. Bishai. (2003) Proc. Natl. Acad. Sci. USA 100:7213-7218. [Abstract/Free Full Text]
Sassetti, C. M., Boyd, D. H. & Rubin, E. J. (2003) Proc. Natl. Acad. Sci. USA 100:12989-12994. [Abstract/Free Full Text]
Lamichhane G, Tyagi S, Bishai WR (2005) Infect Immun. Apr;73(4):2533-40.
[Abstract/Free Full Text]