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HIV-1 Full-length Reporter Infectious Clone
The generation of infectious clones is essential for HIV research. These clones allow researchers to replicate the virus's behavior and investigate its pathogenesis, viral replication process, and host immune responses within a controlled lab setting.
The HIV-1 full-length reporter infectious clone, CCR5-tropic pNL4.3-ADA-GFP-IRES-NEF (HRP-20367), is now available through BEI Resources. This clone is based on the HIV-1 molecular clone pNL4-3 (ARP-114) and includes the GFP-IRES cassette inserted in-frame before the nef gene. The construct encodes all HIV-1 viral proteins, including the CCR5-tropic AdA Env, and co-expresses Nef and GFP from a single bicistronic RNA.1
The virus produced from this construct can infect various cell types, such as CD4+ T lymphocytes, monocyte-derived macrophages, primary fetal microglia, and monocyte-derived microglia. Infected cells show active viral transcription, leading to GFP expression.2,3,4 These cells can be identified using flow cytometry or microscopy, allowing for cell sorting to further characterize the infected cell populations.1
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BEI Resources
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New Reporter Infectious Molecular Clonee
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| HRP-20367 |
HIV-1 Infectious Molecular Clone, pNL4.3-ADA-GFP-IRES-Nef |
References:
1. Cohen, G. B., et al. “The Selective Downregulation of Class I Major Histocompatibility Complex Proteins by HIV-1 Protects HIV-Infected Cells from NK Cells.” Immunity 10 (1999): 661-671. PubMed: 10403641.
2. Pham T. N. Q. , et al. “HIV Nef And Vpu Protect HIV-Infected CD4+ T Cells from Antibody-Mediated Cell Lysis Through Down-Modulation of CD4 And BST2.” Retrovirology 11 (2014): 15. PubMed: 24498878.
3. Bego, M. G., et al. “Vpu Exploits the Cross-Talk between BST2 and the ILT7 Receptor to Suppress Anti-HIV-1 Responses by Plasmacytoid Dendritic Cells.” PLOS Pathog. 11 (2015): e1005024. PubMed: 26172439.
4. Lodge, R., et al. “Host MicroRNAs-221 and -222 Inhibit HIV-1 Entry in Macrophages by Targeting the CD4 Viral Receptor.” Cell Rep. 21 (2017): 141-153. PubMed: 28978468.
Image Alt Text: Image Alt Text: Fluorescence image of TZM-b1 cells infected with purified pNL4.3-ADA-GFP-IRES-NEF virus. GFP expression is shown in green. (BEI Resources)
Image Credit: Image Credit: Fluorescence image of TZM-b1 cells infected with GFP expression shown in green. (BEI Resources)
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