Recombinant Vesicular Stomatitis Virus Expressing SARS-CoV-2 Spike Protein and eGFP

The S glycoprotein remains a primary focus of SARS-CoV-2 immunotherapy research, particularly in the screening of convalescent human plasma and sera for the presence of neutralizing antibodies. Alternative approaches for working with pathogenic viruses outside of a BSL-3 laboratory using recombinant viral systems, such as Vesicular Stomatitis Virus (VSV), have made this research more accessible.

A recombinant VSV recently deposited to BEI Resources by Dr. Kartik Chandran of Albert Einstein College of Medicine resembles SARS-CoV-2 in cellular entry and inhibition through expression of the SARS-CoV-2 S gene as the sole entry glycoprotein. rVSV-SARS-CoV-2 S (BEI Resources NR-55284) was generated by modification of a VSV antigenome, replacing its native glycoprotein with the full-length, wild-type SARS-CoV-2 Wuhan-Hu-1 S gene and enhanced green fluorescent protein (eGFP). After a plasmid-based rescue, rVSV-SARS-CoV-2 S was passaged 9 times and plaque-purified, resulting in a slowly replicating virus expressing the wild-type S gene.1 rVSV-SARS-CoV-2 S is provided as spin-clarified cell lysate and supernatant from infected Cercopithecus aethiops kidney cells (ATCC® CCL-81™).

rVSV-SARS-CoV-2 S is ideal for reporter-based screening of small-molecule and antibody-based inhibitors targeting the SARS-CoV-2 S glycoprotein, such as neutralizing antibodies present in convalescent human plasma and sera, as well as research targeting viral entry and its inhibition.

Please refer to the product documentation located on the BEI Resources website for detailed analysis of eGFP expression, titer determination and recommended growth conditions.


BEI Resources 

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Recombinant Vesicular Stomatitis Virus Expressing SARS-CoV-2 Spike with Enhanced Green Fluorescent Protein






  1. Dieterle, M. E., et al. “A Replication-Competent Vesicular Stomatitis Virus for Studies of SARS-CoV-2 Spike-Mediated Cell Entry and Its Inhibition.” Cell Host Microbe 23 (2020): 486-496. PubMed: 32738193

Image:  Transmission electron microscopic image vesicular stomatitis virus particles. (CDC/Dr. Fred. A. Murphy)

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