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NR-43186   Monoclonal Anti-Rift Valley Fever Virus Gn Glycoprotein, Clone 3C10 (produced in vitro)
(Monoclonal Antibodies)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Monoclonal Anti-Rift Valley Fever Virus Gn Glycoprotein, Clone 3C10 (produced in vitro)
Organism: Rift Valley fever virus
Biosafety Level: 1
Availability Status: In Stock
Store at: -20°C or colder
Contributor: CS Schmaljohn
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Antibody Class: IgG1κ
Mouse monoclonal antibody prepared against the Rift Valley fever virus (RVFV) Gn glycoprotein was affinity purified from clone 3C10 hybridoma supernatant using protein G affinity chromatography. The B cell hybridoma was generated by the fusion of Sp2/0-Ag14 mouse myeloma cells with splenocytes from immunized BALB/c mice. The epitope recognized by the 3C10 antibody is reported to map to amino acids 362-375 of the mature Gn (formerly G2) glycoprotein.

This reagent is part of the Joel M. Dalrymple – Clarence J. Peters USAMRIID Antibody Collection.

Each vial contains approximately 100 µL of purified monoclonal antibody in phosphate-buffered saline (PBS).

NR-43186 is reactive in ELISA using gamma-irradiated RVFV. Clone 3C10 is reported to be reactive in indirect immunofluorescence assays using Vero E6 cells infected with RVFV. The antibody is also reported to function in ELISA as well as radioimmunoprecipitation and western blot assays. The antibody is neither neutralizing in vitro nor protective in mice.
Citations: Acknowledgment for publications should read “The following reagent was obtained from the Joel M. Dalrymple - Clarence J. Peters USAMRIID Antibody Collection through BEI Resources, NIAID, NIH: Monoclonal Anti-Rift Valley Fever Virus Gn Glycoprotein, Clone 3C10 (produced in vitro), NR-43186.”
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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