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NR-48501  Staphylococcus aureus subsp. aureus, Nebraska Transposon Mutant Library (NTML) Screening Array
(Mutant Bacteria)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Organism: Staphylococcus aureus subsp. aureus
Designations: Nebraska Transposon Mutant Library (NTML) Screening Array
Biosafety Level: 2
Availability Status: Temporarily Out of Stock (Please See Comments)
Store at: -60°C or colder
Contributor: K Bayles, P Fey, NARSA
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

This item is currently in our production queue. Please allow ample time for distribution lots to be made available.

The NTML (NTML) Screening Array consists of approximately 2000 Staphylococcus aureus (S. aureus) subsp. aureus USA300 JE2, transposon (Tn) mutants arrayed in five 384-well microtiter plates. The screening array was developed to be a resource for performing high-throughput phenotypic screens to identify candidate genes for future research. The mutants were derived from S. aureus subsp. aureus, strain JE2. Mutagenesis occurred through the use of the mariner-based transposon bursa aurealis resulting in erythromycin resistant deletion strains of JE2. The insertion sites were identified by sequencing the junction fragments containing the end of the transposon and the flanking DNA. The gene names and descriptions associated with each of the Tn mutants were obtained from the National Center for Biotechnology Information.

Note: Please refer to the attached spreadsheet in the additional information section of this website to determine the identity of the specific mutant in each well of each plate.

Each well contains approximately 150 µl of bacterial culture in Tryptic Soy broth containing 5 µg/mL erythromycin supplemented with 25% glycerol.

The Nebraska Transposon Mutant Library (NTML) was constructed in the laboratories of Dr. Ken Bayles and Dr. Paul Fey at the University of Nebraska Medical Center. Additional information is available at the NTML website.

Note: Production in the 384-well format has increased risk of cross-contamination between adjacent wells. Individual clones should be purified (e.g. single colony isolation and purification using good microbiological practices) and sequence-verified prior to use. BEI Resources does not confirm or validate individual clone identities provided by the contributor.
Citations: Acknowledgment for publications should read "The following reagent was provided by the Network on Antimicrobial Resistance in Staphylococcus aureus (NARSA) for distribution by BEI Resources, NIAID, NIH: Nebraska Transposon Mutant Library (NTML) Screening Array, NR-48501."
Additional Information: 384-Well Array Identification
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

Staphylococcus aureus subsp. aureus Nebraska Transposon Mutant Library (NTML) Genetic Toolbox
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BEI Resources products are intended for laboratory research purposes only. They are not intended for use in humans.

While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this site, BEI Resources makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. BEI Resources does not warrant that such information has been confirmed to be accurate.

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