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NR-13299   Plasmid pMRLB.48 Containing Gene Rv2032 (Protein Acg) from Mycobacterium tuberculosis
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Description: Plasmid pMRLB.48 Containing Gene Rv2032 (Protein Acg) from Mycobacterium tuberculosis
Organism: Mycobacterium tuberculosis
Biosafety Level: 1
Availability Status: In Stock
Store at: -20°C or colder
Contributor: NIH - TB Vaccine Testing and Research Materials Contract
Quantity limit for this item is 2 per year. Orders over this limit will be sent to NIAID for approval before shipment.

NR-13299 is a recombinant expression vector containing Mycobacterium tuberculosis gene Rv2032, which encodes a conserved hypothetical protein, Acg. Gene Rv2032 was amplified by PCR and cloned into pET23b for expression in Escherichia coli. The gene was cloned without a signal sequence. The expressed protein is histidine-tagged and has an observed molecular weight of 37 kDa.

Note: Plasmid pMRLB.48 contains the gene required for ampicillin (Ap) resistance. The recommended concentration of Ap in culture is 130 µg/mL.

Each vial contains 1 µg of plasmid DNA in 10 mM Tris-HCl, pH 7.5.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Plasmid pMRLB.48 Containing Gene Rv2032 (Protein Acg) from Mycobacterium tuberculosis, NR-13299."
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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