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NR-29032   Influenza A Virus, A/Puerto Rico/8-CV5/1934 (H1N1)
(Viruses)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Influenza A Virus, A/Puerto Rico/8-CV5/1934 (H1N1)
Organism: Influenza A virus
Biosafety Level: 2
Availability Status: In Stock
Store at: -60°C or colder
Contributor: JW Yewdell
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Influenza A virus, A/Puerto Rico/8-CV5/1934 (H1N1) is a monoclonal antibody escape mutant of influenza A virus, A/Puerto Rico/8/1934 (H1N1). The escape mutant was selected by incubation of parental virus with an over-neutralizing dose of monoclonal antibody (designated "C" by the contributor) that recognizes the influenza A virus hemagglutinin. Incubation was followed by growth of the non-neutralized virus fraction at limiting dilution in the allantois on shell (AOS) culture system. Virus was re-cloned twice by growth at limiting dilution in the absence of antibody, first in the AOS culture system and then in the allantoic cavity of embryonated chicken eggs. The specific amino acid change in the hemagglutinin protein associated with antibody escape mutant CV5 has been described (GenPept: ADX99680).

Sequence information is available for influenza A virus, A/Puerto Rico/8­CV5/1934 (H1N1) at the Influenza Research Database. The virus was originally deposited to BEI Resources as influenza A virus, A/Puerto Rico/8-34-CV5/2010 (H1N1), but subsequently named A/Puerto Rico/8-CV5/1934 (H1N1) by the NIAID Influenza Genome Sequencing Consortium. Please note that the depositor's original nomenclature was used on the product label.

Each vial contains approximately 1 mL of pooled allantoic fluid from specific pathogen free (SPF) embryonated chicken eggs infected with influenza A virus, A/Puerto Rico/8-CV5/1934 (H1N1).
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Influenza A Virus, A/Puerto Rico/8-CV5/1934 (H1N1), NR-29032."
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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