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NR-43001  Murine coronavirus, icA59-ns2dm, Recombinant
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: icA59-ns2dm, Recombinant
Organism: Murine coronavirus
Biosafety Level: 2
Availability Status: In Stock
Store at: -60°C or colder
Contributor: SR Weiss
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval.

NR-43001 is a recombinant murine coronavirus that was produced using a vaccinia virus-based reverse genetics system, and derived from cloned, full-length MHV-A59 cDNA. The icA59-ns2dm virus carries two site-directed mutations in the catalytic region of the cyclic phosphodiesterase ns2, resulting in amino acid substitutions at positions 46 (H46A) and 126 (H126R). Viruses carrying either of these two mutations are attenuated for replication in mouse liver following intrahepatic inoculation, but not in mouse brain following intracranial inoculation. The ns2 phosphodiesterase inhibits the interferon-inducible oligoadenylate synthase (OAS)-RNase L pathway by cleaving 2',5'-oligoadenylate, the product of OAS, preventing activation of the cellular endoribonuclease L, thus blocking viral RNA degradation and facilitating hepatitis development. The complete genome of the recombinant double mutant strain has been sequenced (GenBank: KF268339).

Each vial contains approximately 1 mL of cell lysate and supernatant from mus musculus liver epithelial cells infected with recombinant murine coronavirus, icA59-ns2dm.

Additional information and tools are available at ViPR (Virus Pathogen Resource).
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Recombinant Murine Coronavirus, icA59-ns2dm, NR-43001."
For a list of permits that may be required for shipping this product and to set the permit information preferences; please select a country from the drop down below.

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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