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NR-46419  Staphylococcus aureus, AIS 080003
Price: All BEI Resources products are provided
at no cost to registered researchers.
Organism: Staphylococcus aureus
Designations: AIS 080003
Biosafety Level: 2
Availability Status: In Stock
Store at: -60°C or colder
Contributor: J Patel
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Staphylococcus aureus (S. aureus), strain AIS 080003 (also referred to as VRSA-9 and VRS9) was isolated in 2007 in Michigan, USA from a left plantar foot wound of a 54-year-old female who recently received a 4-week course of vancomycin and levofloxacin to treat osteomyelitis of the left metatarsals.

S. aureus, strain AIS 080003 is a vancomycin-resistant S. aureus strain. S. aureus, strain AIS 080003 was deposited as positive for mec and vanA; negative for vanB, vanC1, vanC2, vanD, vanE, PVL and arginine catabolic mobile element (ACME); pulsed-field type USA100; spa repeats TJMBMDMGMK; Ridom spa type t002. S. aureus, strain AIS 080003 is a USA100 isolate. USA100 isolates have the same MLST profile (ST 5), SCCmec (subtype II) and spa motif (MDMGMK) and are usually resistant to erythromycin and spectinomycin as well as being multiresistant to other commonly used therapeutic agents. USA100 is the most prevalent U.S health care-associated pulse-field type and is endemic in many U.S. hospitals. S. aureus, strain AIS080003 is partially dependent on vancomycin for growth due to a mutation in D-alanyl:D-alanine ligase (Ddl), an enzyme involved in biosynthesis of peptidoglycans. The presence of the vanA operon compensates for the Ddl mutation by providing alternative peptidoglycans for cell wall synthesis; however, some have found that this change in the type of peptidoglycans produced renders it susceptible to oxacillin. The complete genome sequence of S. aureus, strain AIS 080003 is available (GenBank: AHBS00000000).

Each vial contains approximately 0.5 mL of bacterial culture in Brain Heart Infusion broth supplemented with 6 µg/mL vancomycin and 10% glycerol.
Citations: Acknowledgment for publications should read "The following reagent was provided by the Network on Antimicrobial Resistance in Staphylococcus aureus (NARSA) for distribution by BEI Resources, NIAID, NIH: Staphylococcus aureus, Strain AIS 080003, NR-46419."
Additional Information: NARSA VRS9
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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