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BEI Resources Knowledge Base


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The Knowledge Base is an informational database assembled by our Support Specialists and Scientific Staff which provides additional knowledge about the BEI Resources program and reagents through questions and answers. In order to use the Knowledge Base, enter a keyword or phrase that best describes the information of interest. More >>
   

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How do you adapt an egg grown Influenza virus to cell culture?
If adapting influenza virus from one propagation host to another propagation host, it is important to passage the virus at least three times in the new host to allow for adaptation. Each passage should be tested for virus growth, and it may also be important to check the titer of each passage to ensure that the virus is not being lost during adaptation to the new host. When changing the propagation host, it is also important to consider changes that may need to be made to the viral growth medium, temperature and cell confluency (if appropriate) based on the properties of the new host. For propagating influenza virus in tissue culture, the viral growth medium should not contain Fetal Bovine Serum (FBS), as serum will neutralize the activity of TPCK-treated trypsin.
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Updated on: 10/29/2014 2:56:37 PM
What is the importance of testing TPCK-treated trypsin lots from the same vendor when using it as a supplement in influenza virus growth in MDCK cells? How should it be tested?
TPCK-treated trypsin is available from several manufacturers. It is available in varying concentrations from different manufacturers, and some manufacturers offer the product based on specific activity, not concentration. Additionally, concentration will depend on purity. Therefore, it is important to determine the appropriate concentration of each lot of TPCK-trypsin used in the lab. To determine the appropriate concentration, the host cells should first be at 100% confluency before TPCK-treated trypsin is added. Then various concentrations should be tested on the 100% confluent host cell monolayer to determine the highest concentration that can be added to the cells without causing cytotoxicity to the host cell monolayer for the duration of the incubation period needed for growth of the virus. This testing should be conducted for each cell line that may be used with TPCK-treated trypsin to grow influenza virus, and must be repeated with each new lot of TPCK-treated trypsin.
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Updated on: 10/29/2014 2:53:55 PM
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Showing 1 to 5 of 9 Results12
SARS Virus Now Available for Researchers
We have available for the first time in the BEI Resources catalog, two SARS Coronaviruses (SARS-CoV) derived from the Urbani strain. NR-18925 is produced from a recombinant infectious clone, icSARS-CoV. NR-15418 is the mouse-adapted strain, Urbani v2163, developed by Dr. Barnard as a lethal model fo ...
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Updated on: 12/4/2018 2:36:38 PM
Sand Flies, Reduviids and Ticks are Here!
BEI Resources announces the addition of Sand Flies, Reduviids and Ticks for the research community. Catalog Number Vector Pathogen Competence   NR-43999 Phlebotomus papatasi, PPNS, Larval Leishmania major and Sand Fly Fever phleboviruses.   NR-44000 Phlebotomus papata ...
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Updated on: 12/4/2018 2:32:27 PM
Development of let digital PCR for the detection of Babesia microti and Babesia duncani
Development of let digital PCR for the detection of Babesia microti and Babesia duncani Babesia spp. are obligate protozoan parasites of red blood cells. Transmission to humans occurs through bites from infected ticks or blood transfusion. Infections with B. microti account for the majority of the ...
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Updated on: 12/9/2014 10:58:36 AM
Mycobacterium tuberculosis Production Manuals & SOP's from Dr. Karen Dobos
Please use this link  to find a comprehensive list of  protocols and SOPs for Mycobacterium tuberculosis including: Large Scale Growth Subcellular Fractions Preparation and Fractionation of Cell Wall Proper Lipoarabinomannan (LAM) and Lipomannan (LM) Antibodies Microarrays Media Native Ma ...
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Updated on: 11/20/2014 11:18:21 AM
BEI Resources Fungi Catalog is Growing!
  BEI Resources is working to expand the fungal catalog so that researchers have a broader range of organisms and reagents for their work.  BEI Resources currently has eight genus and species represented with several more currently going through the production pipeline.  BEI Resources i ...
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Updated on: 8/29/2014 9:49:43 AM
Showing 1 to 5 of 9 Results12
The following is a list of published abstracts and papers where the work was either facilitated, or directly supported, by the BEI Resources Program.


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Santa Maria, F., et al., Inactivation of SARS-CoV-2 in All Blood Components Using Amotosalen/Ultraviolet A Light and Amustaline/Glutathione Pathogen Reduction Technologies. Pathogens, 2022. 11(5): p. 521.
Author: BEI Resources
Updated on: 5/3/2022 12:26:39 PM
Walker, A.C., et al., Bacteria-Derived Protein Aggregates Contribute to the Disruption of Host Proteostasis. International Journal of Molecular Sciences, 2022. 23(9): p. 4807.
Author: BEI Resources
Updated on: 5/3/2022 12:23:48 PM
Kalkal, M., et al., IL-10 Producing Regulatory B Cells Mediated Protection against Murine Malaria Pathogenesis. Biology, 2022. 11(5): p. 669.
Author: BEI Resources
Updated on: 5/3/2022 12:22:29 PM
de Vries, L.E., et al., Preclinical characterization and target validation of the antimalarial pantothenamide MMV693183. Nature Communications, 2022. 13(1): p. 2158.
Author: BEI Resources
Updated on: 5/3/2022 12:22:01 PM
Faísca, F., et al., Enhanced In Vitro Antiviral Activity of Hydroxychloroquine Ionic Liquids against SARS-CoV-2. Pharmaceutics, 2022. 14(4): p. 877.
Author: BEI Resources
Updated on: 5/3/2022 12:21:31 PM
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