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All Anopheles
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Wild Stocks
Strain Organism MRA No. Origin Notes
G3
A. gambiae
112
Gambia
wild-type stock that has been in culture for over 30 years. It is considered a standard research organism, TEP1 s/s, G3 is primarily a Savanna type stock
ASEMBO1
A. gambiae
186
Kenya
This strain was recently found to have a potential translocation of the X chromosome onto the Y chromosome
MOPTI
A. gambiae
763
Mali
This is a wild-type Mopti rDNA form stock.
KISUMU1
A. gambiae
762
Kenya
This strain is considered the insecticide susceptible standard
STE2
A. stephensi
128
India
This strain is often used in Plasmodium berghei studies
EBRO
A. atroparvus
493
Spain
wild type
F1
A. freeborni
130
United States
wild type, wild eye and body color,excellent sporozoite-producer strain
FAR1
A. farauti
489
Papua New Guinea
Infects well with most Plasmodium species. An. farauti no. 1
KGB
A. arabiensis
339
Zimbabwe
This strain is considered a standard research organism
DONGOLA
A. arabiensis
856
Sudan
This strain was created for the SIT program in Sudan
ORLANDO
A. quadrimaculatus
139
United States
wild type colony. An. quadrimaculatus A
STECLA
A. albimanus
126
El Salvador
This stock was developed during the SIT program in El Salvador
SKUQUA
A. quadriannulatus
761
South Africa
wild type, A. gambiae complex member
WRAIR2
A. dirus
700
Thailand
force mated species. A. dirus A
MINIMUS1
A. minimus
729
Thailand
wild type colony. A. minimus A
OPHANSI
A. merus
803
South Africa
wild-type, A. gambiae complex member
PIMPERENA
A. gambiae
861
Mali
This strain was used in the recent genome project (www.vectorbase.org) and is the Savanna rDNA form standard
MALI-NIH
A. gambiae
860
Mali
This strain was used in the recent genome project (www.vectorbase.org) and is the Mopti rDNA form standard

Insecticide Resistance
Strain Organism MRA No. Insecticide/ Origin Notes Thumbnail
ZAN/U
A. gambiae
594
DDT/ Zanzibar
 
This is a DDT-resistant stock. ZAN/U expresses both a glutathione-S-transferase mechanism and elevated beta-esterase activity
  
RSP
A. gambiae
334
Permethrin/ Kenya
 
This strain expresses both the L1014S KDR mechanism and increased cytochrome P450 activity
RSP-ST
A. gambiae
698
Permethrin/ Kenya
 
This strain was selected from RSP for a standard karyotype. Unlike RSP, this strain only expresses the L1014S KDR mechanism
IN22C+
A. gambiae
115
Dieldrin
 
Dieldrin resistant due to GABA mutation (Ala296Gly) combined with a pericentric inversion on chromosome 2
P+DLRC+R+
A. gambiae
116
Dieldrin
 
Dieldrin resistant due to GABA mutation (Ala296Gly)
SENN
A. arabiensis
764
Dieldrin/ Sudan
 
Dieldrin resistant due to GABA mutation (Ala296Ser)

Transgenic
Strain Organism MRA No. Notes Thumbnail
P-BAC-0
A. gambiae
335
eGFP expression particularly in the anterior stomach and distal lobes of salivary glands; Contains pK[BIGalpha] insertion in 28D. See Genbank accession numbers AF402294 and AF402295
IV
A. stephensi
314
L4s of this stock showed GFP expression primarily in gastric cecae and the posterior stomach
SRtTA
A. stephensi
728
SRtTA is homozygous for Actin 5C-eGFP marker with an autosomal insertion of a reverse tTA which is controlled by a serpin 10 promoter fragment. Progeny crosses to a LacZ reponder line reveal staining in the midgut, hemocytes and pericardial cells of larvae and adults.
StTA/ tetOPLacZ
A. stephensi
727
This stock has an X-linked tetracycline transactivator gene driver (tTA1) which is controlled by a seprin 10 promoter fragment. Regulation of this system is done via doxycycline. This strain is double marked with an Actin 5c-eGFP and an Actin 5C-dsRed1 marker. Lines carrying individual insertions should be isolated following outcrossing to wild type A. stephensi.
B1
A. stephensi
855
RNA silencing of eGFP transcripts in transgenic A. stephensi mosquitoes. pIR-EGFP insertion with dsRed marker under Actin5C promoter. Parental crosses result in 43% reduction in GFP expression. Backcrosses of progeny to B1 males results in 73% reduction in expression.
19.1
A. stephensi
854
RNA silencing of eGFP transcripts in transgenic A. stephensi mosquitoes. pIR-EGFP insertion with dsRed marker under Actin5C promoter. Parental crosses result in a 37% reduction in GFP expression. Backcrosses of progeny to B1 males results in a 65% reduction in expression.

Encapsulation Effect
Strain Organism MRA No.

Notes

 Thumbnail
L3-5
A. gambiae
114
This strain has the unique property of being selected for a hyper-reactive immune reaction to infection with Plasmodium cynomolgi B. When infected, >95% of oocysts will be encapsulated within the midgut when compared to 4ARR (MRA-121). This stock is also fixed for the TEP1 allele which may confer resistance to P. berghei infection
4ARR
A. gambiae
121
This strain has the unique property of being selected for a lack of immune reaction to Plasmodium cynomolgi B infection. When infected, >95% of oocysts in the midgut are not encapsulated when compared to L3-5 (MRA-114). 4ARR also has a prominent pumpkin eye color mutation marker

Genetic Marker Strains
Strain Organism MRA No. Body Color Eye Color Notes Thumbnail
M2
A. gambiae
105
wild
white
contains an X-linked mutation in the white allele
RMOSPW
A. gambiae
111
wild
mosaic
duplicated 2Rb pink-eye allele and an X-linked white allele mutation
GORO
A. quadrimaculatus
891
golden
dark pink
This strain was created by combining 2 X-linked mutations (rose eye and golden cuticle)

 
SUA2LA
A. gambiae
765
wild
wild
This stock is the standard A. gambiae strain in Europe for genetic mapping, in situ hybridizations, and behavioral studies
 

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