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This plasmid is derived from pMH94 (Lee and Hatfull, 1991. PNAS) from which the DraI-FspI fragment was removed and ligated to the Invitrogen Gateway system reading frame (rfa_verB). This plasmid allows for efficient transfer of DNA fragments from Gateway entry vectors. This plasmid has been optimized to deliver DNA for stable integration at the mycobacterial attB locus.