TARGET Research Results
Details
DescriptionPROBABLE TRANSMEMBRANE SERINE/THREONINE-PROTEIN KINASE I PKNI (PROTEIN KINASE I) (STPK I) (PHOSPHORYLASE B KINASE KINASE) (HYDROXYALKYL-PROTEIN KINASE)
Accession numberRv2914c
Gene namepknI
Gene length1758
Protein length585
Coordinates3221767..3223524
Orientation-
Molecular mass61804.65
Isoelectric point5.63

Database Crossreferences
Deletion Mutant Phenotype
in-vivoguinea pig
 mouse
 rabbit

in-vitroHBMEC
 macrophage

Mutant Survival in DeADMAn (Lamichhane et al., 2005)*

Strain NamePhenotype (Mouse)
JHU2914c-896[no data]
JHU2914c-134[no data]
JHU2914c-43Not-Attenuated
JHU2914c-133[no data]
 

CDC1551 Homolog
ORFMT2982
Essentiality Prediction
Sassetti et al, 2003*non-essential

JHU Transposon Mutant(s) Available
NameMT#Rv#POIGene LengthAvailable from BEI
JHU2914c-896MT2982Rv2914c8961812no (will be available later)
JHU2914c-134MT2982Rv2914c1341812yes
JHU2914c-133MT2982Rv2914c1331812yes
  
For a list of currently available mutants, please visit BEI's transposon mutant webpage.

If we have not disrupted your gene using random mutagenesis, we may be able to create a knockout mutant for you.

References
Lamichhane, G., M. Zignol, N. J. Blades, D. E. Geiman, A. Dougherty, J. Grosset, K. W. Broman, and W. R. Bishai. (2003) Proc. Natl. Acad. Sci. USA 100:7213-7218. [Abstract/Free Full Text]
Sassetti, C. M., Boyd, D. H. & Rubin, E. J. (2003) Proc. Natl. Acad. Sci. USA 100:12989-12994. [Abstract/Free Full Text]
Lamichhane G, Tyagi S, Bishai WR (2005) Infect Immun. Apr;73(4):2533-40.
[Abstract/Free Full Text]