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Antibody class: IgG1κ
ARP-857 is purified from hybridoma supernatant by protein G affinity chromatography and quantitated by ELISA.
This antibody binds to a discontinuous, or conformational gp120 epitope.
This antibody binds to gp120 on the surface of IIIB, SF2, MN, RF, and CC-infected cells. It neutralizes SF2, IIIB, and MN infection at concentrations ranging from 140 ng/mL to 10 µg/mL.
The hybridoma is generated using human Epstein-Barr Virus-transformed B cells fused with HMMA2.11TG/O heteromyeloma.
Note: The user should determine the optimal concentration for any application.
Each vial of ARP-857 contains 100 µg of purified antibody at a concentration of 1.5 mg/mL, dialyzed against PBS and sterile-filtered. Please refer to the attached file(s) for additional information.
The endotoxin content is 340 EU/mL for lot 160077.
If lower endotoxin is required for your needs, please request Lot No. 150350 in your order. *NOTE: Endotoxin has been shown to induce chemokines and other soluble HIV and SIV inhibitory factors in primary cultures of PBMC, monocytes and macrophages (Verani et al., J. Exp. Med. 185: 805-816, 1997; J. Immunol. 168: 6388-6396, 2002; Montefiori in preparation). Thus, caution is advised when using this reagent for in vitro studies involving primary cells. Immunoreactivity is confirmed by ELISA and purity is assessed by SDS-PAGE analysis.Click Here for Purity and ELISA Data.
Note: Avoid freeze-thaw cycles as reagent degradation may result.
Cavacini, L. A., et al. “Human Monoclonal Antibodies to the V3 Loop of HIV-1 gp120 Mediate Variable and Distinct Effects on Binding and Viral Neutralization by a Human Monoclonal Antibody to the CD4 Binding Site.” J. Acquir. Immune Defic. Syndr. 6 (1993): 353-358. PubMed: 8455141.
Posner, M. R., et al. “Neutralization of HIV-1 by F105, a Human Monoclonal Antibody to the CD4 Binding Site of gp120.”J Acquir. Immune. Defic. Syndr. 6 (1993): 7-14. PubMed: 8417177. Posner, M. R., H. Elboim, and D. Santos. “The Construction and Use of a Human-Mouse Myeloma Analogue Suitable for the Routine Production of Hybridomas Secreting Human Monoclonal Antibodies.” Hybridoma 6 (1987): 611-625. PubMed: 3436625.
Posner, M. R., et al. “An IgG Human Monoclonal Antibody that Reacts with HIV-1/gp120, Inhibits Virus Binding to Cells, and Neutralizes Infection.” J. Immunol. 146 (1991): 4325-4332. PubMed: 1710248.
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