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BEI Resources currently limits the total number of "Made to Order" items which can be ordered by a registrant to 10 "Made to Order" items per 6 month period. Please check the Availability Status on the items you are ordering and limit orders appropriately.
NR-20083  Influenza A (H1N1) 2009 Monovalent Vaccine Influenza A virus
(Vaccines)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Influenza A virus
Organism: Influenza A (H1N1) 2009 Monovalent Vaccine
Biosafety Level: 1
Availability Status: In Stock
Store at: 2°C to 8°C
Contributor: NIAID/NIH
Comments:
Quantity limit per order for this item is 3. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Influenza A (H1N1) 2009 (pdm09) Monovalent Vaccine is a subunit (purified surface antigen) influenza virus vaccine prepared from A/California/7/2009 (H1N1)pdm09-like virus propagated in the allantoic cavity of embryonated chicken eggs. The virus was harvested and clarified by centrifugation and filtration prior to inactivation with betapropiolactone. The inactivated virus was concentrated and purified by zonal centrifugation. The surface antigens, hemagglutinin and neuraminidase, were obtained from the influenza virus particle by further centrifugation in the presence of nonylphenol ethoxylate, a process which removes most of the internal proteins. The nonylphenol ethoxylate was removed from the surface antigen preparation. Thimerosal, a mercury derivative used during manufacture, was removed by the purification steps to a trace amount (≤ 1 µg mercury per dose). Each 0.5 mL dose may also contain residual amounts of egg proteins (≤ 1 µg ovalbumin), polymyxin (≤ 3.75 µg), neomycin (≤ 2.5 µg), betapropiolactone (≤ 0.5 µg) and nonylphenol ethoxylate (≤ 0.015% w/v).

The hemagglutinin content was standardized according to U.S. Public Health Service requirements. Each 0.5 mL syringe contains the recommended 15 µg of hemagglutinin antigen.

Please note that this vaccine preparation is being released for research use only and not for human use. Vaccines produced for the 2009-2010 season are now past their expiration dates.

Each syringe contains 0.5 mL of monovalent vaccine in phosphate-buffered saline.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources Repository, NIAID, NIH: Influenza A (H1N1) 2009 Monovalent Vaccine, NR-20083."
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