TARGET Research Results
Details
DescriptionPROBABLE METHYLMALONYL-CoA MUTASE LARGE SUBUNIT MUTB (MCM)
Accession numberRv1493
Gene namemutB
Gene length2253
Protein length750
Coordinates1684005..1686257
Orientation+
Molecular mass80603.93
Isoelectric point5.69

Database Crossreferences
Deletion Mutant Phenotype
in-vivoguinea pig
 mouse
 rabbit

in-vitroHBMEC
 macrophage

Mutant Survival in DeADMAn (Lamichhane et al., 2005)*

Strain NamePhenotype (Mouse)
JHU1493-284[no data]
JHU1493-1268[no data]
JHU1493-184[no data]
JHU1493-1271[no data]
 

CDC1551 Homolog
ORFMT1540
Essentiality Prediction
Sassetti et al, 2003*non-essential

JHU Transposon Mutant(s) Available
NameMT#Rv#POIGene LengthAvailable from BEI
JHU1493-1268MT1540Rv149312682253yes
JHU1493-184MT1540Rv14931842253yes
JHU1493-1271MT1540Rv149312712253no (will be available later)
  
For a list of currently available mutants, please visit BEI's transposon mutant webpage.

If we have not disrupted your gene using random mutagenesis, we may be able to create a knockout mutant for you.

References
Lamichhane, G., M. Zignol, N. J. Blades, D. E. Geiman, A. Dougherty, J. Grosset, K. W. Broman, and W. R. Bishai. (2003) Proc. Natl. Acad. Sci. USA 100:7213-7218. [Abstract/Free Full Text]
Sassetti, C. M., Boyd, D. H. & Rubin, E. J. (2003) Proc. Natl. Acad. Sci. USA 100:12989-12994. [Abstract/Free Full Text]
Lamichhane G, Tyagi S, Bishai WR (2005) Infect Immun. Apr;73(4):2533-40.
[Abstract/Free Full Text]