TARGET Research Results
Details
DescriptionProbable exported protease
Accession numberRv2224c
Gene nameRv2224c
Gene length1563
Protein length520
Coordinates2495461..2497023
Orientation-
Molecular mass55892.22
Isoelectric point6.07

Database Crossreferences
Deletion Mutant Phenotype
in-vivoguinea pig
 mouse
 rabbit

in-vitroHBMEC
 macrophage

Mutant Survival in DeADMAn (Lamichhane et al., 2005)*

Strain NamePhenotype (Mouse)
JHU2224c-197[no data]
JHU2224c-1075[no data]
JHU2224c-1076[no data]
JHU2224c-862Attenuated
JHU2224c-986[no data]
 

CDC1551 Homolog
ORFMT2282
Essentiality Prediction
Sassetti et al, 2003*non-essential

JHU Transposon Mutant(s) Available
NameMT#Rv#POIGene LengthAvailable from BEI
JHU2224c-197MT2282Rv2224c1971524yes
JHU2224c-1075MT2282Rv2224c10751524yes
JHU2224c-986MT2282Rv2224c9861524yes
  
For a list of currently available mutants, please visit BEI's transposon mutant webpage.

If we have not disrupted your gene using random mutagenesis, we may be able to create a knockout mutant for you.

References
Lamichhane, G., M. Zignol, N. J. Blades, D. E. Geiman, A. Dougherty, J. Grosset, K. W. Broman, and W. R. Bishai. (2003) Proc. Natl. Acad. Sci. USA 100:7213-7218. [Abstract/Free Full Text]
Sassetti, C. M., Boyd, D. H. & Rubin, E. J. (2003) Proc. Natl. Acad. Sci. USA 100:12989-12994. [Abstract/Free Full Text]
Lamichhane G, Tyagi S, Bishai WR (2005) Infect Immun. Apr;73(4):2533-40.
[Abstract/Free Full Text]