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Bioluminescent transgenic parasites for Toxoplasmosis Research
Toxoplasma gondii, the causal agent of toxoplasmosis, is an ideal model system to study the basic biology of parasitism. Recent additions to the BEI Resources protozoan catalog include two bioluminescent transgenic T. gondii reporter strains derived from the type I strain RH (NR-59752) and the type II strain ME49 (NR-59751). These engineered strains, expressing nanoluciferase, may be used in drug screen assays in vitro as well as in vivo in combination with imaging systems to track infection spread, tissue distribution, and reactivation in laboratory mice.
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BEI Item No.
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Description
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| NR-59751 |
Toxoplasma gondii ME49 FLUC |
| NR-59752 |
Toxoplasma gondii RH FLUC |
References:
1. Tobin C. M. and L. J. Knoll. “A Patatin-Like Protein Protects Toxoplasma gondii from Degradation in a Nitric Oxide-Dependent Manner.” Infect. Immun. 80 (2012): 55-61. PubMed: 22006568.
2. Radke J. B. et al. “Bicyclic Azetidines Target Acute and Chronic Stages of Toxoplasma gondii by Inhibiting Parasite Phenylalanyl T-RNA Synthetase.” Nat. Commun. 13 (2022): 459. PubMed: 35075105.
Image Alt Text: Human foreskin fibroblasts infected with Toxoplasma gondii. Infected cells show multiple intracellular parasitophorous vacuoles with tachyzoite forms of the parasite. Image taken at 1000X magnification. Credit: BEI Resources
Image Credit: Human foreskin fibroblasts infected with Toxoplasma gondii. Credit: BEI Resources
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