Azole-Resistant and Dual-Fluorescent Strains of Candida spp.
Highlights

C. albicans and C. glabrata are ubiquitous in the environment and commensal inhabitants of the normal flora of the oral cavity, gastrointestinal tract and skin of most healthy humans.1,2 For the immunocompromised, however, C. albicans and C. glabrata are the two most commonly recovered pathogenic yeasts in the United States and together are responsible for approximately 70% of all cases of systemic candidiasis, with increasing occurrences of multidrug resistance.1-4

BEI Resources houses over 50 strains of Candida spp. to support candidiasis research. Recent additions to our catalog include an azole-susceptible strain of C. glabrata (NR-51685) and an azole-resistant C. glabrata strain isolated from the same patient after 50-day azole therapy (NR-51686).4 Also newly available is a transgenic strain of C. albicans (NR-51634) that constitutively expresses green fluorescent protein (GFP) and red fluorescent protein via mCherry, which has been used as an in vitro system for fluorescent sorting of C. albicans with macrophages.1

For more information about these and other Candida strains available from BEI Resources, or to contribute to our expanding catalog of biomaterials for fungal research, please contact us at Contact@BEIResources.org.

BEI Resources No. Product Description
NR-51685 Candida glabrata, Strain DSY562 - Azole-Susceptible
NR-51686 Candida glabrata, Strain DSY565 - Azole-Resistant
NR-51634 Candida albicans, Strain CAI4-F2-Neut5L-NAT1-mCherry-GFP

 

References:

  1. Muñoz, J. F., et al. “Coordinated Host-Pathogen Transcriptional Dynamics Revealed Using Sorted Subpopulations and Single Macrophages Infected with Candida albicans.” Nat. Commun. 10 (2019): 1607. PubMed: 30962448.
  2. Brunke, S. and B. Hube. “Two Unlike Cousins: Candida albicans and C. glabrata Infection Strategies.” Cell. Microbiol. 15 (2013): 701-708. PubMed: 23253282.
  3. Hendrickson, J. A., et al. “Antifungal Resistance: A Concerning Trend for the Present and Future.” Curr. Infect. Dis. Rep. 21 (2019): 47. PubMed: 31734730.
  4. Vale-Silva, L., et al. “Comparative Genomics of Two Sequential Candida glabrata Clinical Isolates.” G3 (Bethesda) 7 (2017): 2413-2426. PubMed: 28663342.

Image:  Fluorescence microscopy of transgenic Candida albicans, NR-51634 (BEI Resources)

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