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NR-14686   Yersinia pestis Multiplex PCR Primers for Plasmid Detection
(Assays/Panels)
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at no cost to registered researchers.
Description: Yersinia pestis Multiplex PCR Primers for Plasmid Detection
Organism: Yersinia pestis
Biosafety Level: 1
Availability Status: In Stock
Store at: -20°C or colder
Contributor: NIH Biodefense and Emerging Infections Research Resources Repository
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Yersinia pestis organisms may carry three plasmids that contribute to pathogenicity in addition to chromosomal virulence factors on the pgm locus. The pPCP1 (pPla) plasmid contains genes that encode the pesticin immunity protein and the plasminogen activator, which promote bacterial dissemination. The pMT1 (pFra1) plasmid, encodes capsular fraction 1 protein which allows evasion of phagocytosis. The pCD1 (pYV) plasmid encodes the low-calcium response V-antigen, which is part of the type III secretion system involved in directing bacterial proteins to the host cell cytosol. The presence of pCD1 is required for full virulence and, together with the pgm locus, classifies Yersinia pestis as a select agent.

NR-14686 consists of a mixture of forward and reverse primers that are designed to detect the pPCP1, pMT1 and pCD1 plasmids using standard polymerase chain reactions, resulting in amplicons of approximately 400, 1200, and 1900 base pairs, respectively. The primer mixture also contains a positive control primer set that amplifies an approximately 800 base pair chromosomal marker unique to Yersinia pestis. Each primer set is available individually by requesting the appropriate BEI Resources Component number (below). Additionally, a positive control (BEI Resources NR-2715, Genomic DNA from Yersinia pestis, Strain ZE94-2122) that contains all three plasmids, and a negative control (BEI Resources NR-2646, Genomic DNA from Yersinia enterocolitica subsp. enterocolitica, Strain 33114) that contains none of the plasmids are available by requesting the appropriate BEI Resources Related Product number (below).

NR-14686 contains approximately 300 µL of a mixture of forward and reverse primers in TE buffer (pH 7.0).
Components: NR-9686,NR-9687,NR-9688,NR-9689
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Yersinia pestis Multiplex PCR Primers for Plasmid Detection, NR-14686."
PERMITS
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

RELATED PRODUCTS
NR-2646
Genomic DNA from Yersinia enterocolitica subsp. enterocolitica, Strain 33114
NR-2715
Genomic DNA from Yersinia pestis, Strain ZE94-2122
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