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NR-15577   Swine Influenza A (H1N1) Real-Time RT-PCR Assay
(Assays/Panels)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Swine Influenza A (H1N1) Real-Time RT-PCR Assay
Organism: Influenza A virus
Biosafety Level: 1
Availability Status: In Stock
Store at: -20°C or colder
Contributor: BEI Resources
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

The Swine Influenza A (H1N1) Real-Time RT-PCR Assay includes oligonucleotide primers and dual-labeled hydrolysis (TaqMan®) probes to be used in real-time RT-PCR assays for the in vitro qualitative detection and characterization of swine influenza viruses. The TaqMan® probes are labeled with the reporter molecule 6-carboxyfluorescein (6-FAM) and with the quencher, Black Hole Quencher™ 1 (BHQ1).

NR-15577 contains the following components: 1) A Universal Influenza A primer and probe set designed to detect type A influenza viruses, 2) A Swine Influenza A primer and probe set designed to detect swine influenza A viruses, 3) A Swine Influenza H1 primer and probe set designed to detect swine H1 influenza A viruses, 4) A Human RNase P Positive Control primer and probe set designed to detect the presence of RNA from the human RNase P gene in human samples, indicating acceptable sample quality, 5) A Swine Influenza A (H1N1) Positive Control containing PBS spiked with beta-propiolactone (BPL)-inactivated swine H1N1 influenza A virus and human epithelial cells, 6) A Novel Influenza A (H1N1) Positive Control containing PBS spiked with BPL-inactivated novel H1N1 influenza A virus and human epithelial cells, and 7) A Human Specimen Extraction Control containing PBS spiked with human epithelial cells.

Primers are provided lyophilized at approximately 20 nmoles per vial. Probes are provided lyophilized at approximately 5 nmoles per vial. Primers and probes should be reconstituted with 500 µL of nuclease-free water. The Positive Controls and the Extraction Control are provided in 500 µL volumes.
Components: NR-15578,NR-15579,NR-15580,NR-15581,NR-15582,NR-15583,NR-15584,NR-15585,NR-15586,NR-15587,NR-15588,NR-15589,NR-15590,NR-15591,NR-15627
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Swine Influenza A (H1N1) Real-Time RT-PCR Assay, NR-15577."
PERMITS
For a list of permits that may be required for shipping this product and to set the permit information preferences; please select a country from the drop down below.

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

RELATED PRODUCTS
NR-15592
Influenza A & B viruses Influenza Virus Real-Time RT-PCR Assay
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