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NR-15592   Influenza Virus Real-Time RT-PCR Assay
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Influenza Virus Real-Time RT-PCR Assay
Organism: Influenza A & B viruses
Biosafety Level: 1
Availability Status: In Stock
Store at: -20°C or colder
Contributor: BEI Resources
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

The Influenza Virus Real-Time RT-PCR includes oligonucleotide primers and dual-labeled hydrolysis (TaqMan®) probes to be used in real-time RT-PCR assays for the in vitro qualitative detection and characterization of seasonal (A/H1, A/H3 and B) and A/H5 (asian-lineage) influenza viruses. The TaqMan® probes are labeled with the reporter molecule 6-carboxyfluorescein (6-FAM) and with the quencher, Black Hole Quencher™ 1 (BHQ1).NR-15592 contains the following components: 1) an influenza A primer and probe set designed to detect type A influenza viruses, 2) an influenza H1 primer and probe set designed to detect H1 influenza A viruses, 3) an influenza H3 primer and probe set designed to detect H3 influenza A viruses, 4) an influenza H5a primer and probe set designed to detect H5a influenza A viruses, 5) an influenza H5b primer and probe set designed to detect H5b influenza A viruses, 6) an influenza B primer and probe set designed to detect influenza B viruses, 7) a human RNase P positive control primer and probe set designed to detect the presence of RNA from the human RNase P gene in human samples, indicating acceptable sample quality, 8) a Human Seasonal Influenza Virus Positive Control containing PBS spiked with beta-propiolactone (BPL)-inactivated seasonal influenza viruses (A/H1, A/H3 and B) and human epithelial cells, 9) an Influenza A/H5 (Asian-Lineage) Positive Control containing PBS spiked with formalin-inactivated, detergent-disrupted and genetically modified reassortant influenza virus (A/H5) and human epithelial cells, and 10) a Human Specimen Extraction Control containing PBS spiked with human epithelial cells.

Primers are provided lyophilized at approximately 20 nmoles per vial. Probes are provided lyophilized at approximately 5 nmoles per vial. Primers and probes should be reconstituted with 500 µL of nuclease-free water. The Positive Controls and the Extraction Control are provided in 500 µL volumes.
Components: NR-15593,NR-15594,NR-15595,NR-15596,NR-15597,NR-15598,NR-15599,NR-15600,NR-15601,NR-15602,NR-15603,NR-15604,NR-15605,NR-15606,NR-15607,NR-15608,NR-15609,NR-15610,NR-15611,NR-15612,NR-15613,NR-15614,NR-15615,NR-15616
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Influenza Virus Real-Time RT-PCR Assay, NR-15592."
For a list of permits that may be required for shipping this product and to set the permit information preferences; please select a country from the drop down below.

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

Influenza A virus Swine Influenza A (H1N1) Real-Time RT-PCR Assay
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