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NR-35520  Rickettsia sp., Pan-Rickettsia Quantitative PCR (qPCR) Assay Detection Kit
(Assays/Panels)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Pan-Rickettsia Quantitative PCR (qPCR) Assay Detection Kit
Organism: Rickettsia sp.
Biosafety Level: 1
Availability Status: In Stock
Store at: -20°C or colder
Contributor: BEI Resources
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

The Pan-Rickettsia Quantitative PCR Assay Detection Kit (NR-35520) is designed to detect and quantitate the presence of Rickettsia species. The assay consists of the following components:

1) Forward and reverse primers (NR-35354 and NR-35355, respectively)
2) Probe designed with 6-carboxyfluorescein (6-FAM) at the 5' end and both a minor groove binding moiety (MGB) and a non-fluorescent quenching dye (NFQ) at the 3' end (NR-35356)
3) Plasmid-based standard containing Rickettsia prowazekii ribosomal RNA (rRNA) gene sequences (NR-35519)

Each vial of primer contains 200 µL in 0.2 mM Tris, 0.02 mM EDTA, pH 8.0. Each vial of probe contains 200 µL in 0.5 mM Tris, 0.05 mM EDTA, pH 8.0. Each vial of plasmid-based standard contains 2 × 1010 molecules in 100 µL nuclease-free water.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Pan-Rickettsia Quantitative PCR (qPCR) Assay Detection Kit, NR-35520."
PERMITS
For a list of permits that may be required for shipping this product and to set the permit information preferences; please select a country from the drop down below.

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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