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NR-9350  Orthopoxvirus, Pan-Orthopox Virus E9L Gene-Specific Quantitative PCR Assay Detection Kit
(Assays/Panels)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Pan-Orthopox Virus E9L Gene-Specific Quantitative PCR Assay Detection Kit
Organism: Orthopoxvirus
Biosafety Level: 1
Availability Status: In Stock
Store at: -60°C or colder
Contributor: BEI Resources
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

The Pan-Orthopox Virus E9L Gene-Specific Quantitative PCR Assay Detection Kit (NR-9350) is designed to detect and quantitate the presence of orthopox viruses. The assay was developed using a segment of the E9L gene from vaccinia virus, New York City Board of Health (NR-54) and consists of the following components.

1) Probe designed with 6-carboxyfluorescein (6-FAM) at the 5' end and both the minor groove binder (MGB) and a non-fluorescent quenching dye at the 3' end (NRC-1325; available individually as NR-9344)
2) Forward and reverse primers (NRC-1326 and NRC-1327; available individually as NR-9345 and NR-9346, respectively)
3) Linearized plasmid-based standard containing a segment of the E9L gene derived from vaccinia virus in a commercial vector (NRC-1324; available individually as NR-9343)

Each vial of primer and probe contains 90 to 100 µL in TE buffer (pH 7.0). Each vial of plasmid-based standard contains approximately 45 ng in TE buffer (pH 7.0).
Components: NRC-1324,NRC-1325,NRC-1326,NRC-1327
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Pan-Orthopox Virus E9L Gene-Specific Quantitative PCR Assay Detection Kit, NR-9350."
PERMITS
For a list of permits that may be required for shipping this product and to set the permit information preferences; please select a country from the drop down below.

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

RELATED PRODUCTS
NR-54
Vaccinia virus New York City Board of Health (Wyeth, Calf Adapted)
NR-9343
Plasmid Containing a Segment of the E9L Gene from Vaccinia Virus, New York City Board of Health, Linearized
NR-9344
Orthopoxvirus Pan-Ortho Pox E9L Gene-Specific Quantitative PCR Probe
NR-9345
Orthopoxvirus Pan-Orthopox Virus E9L Gene-Specific Quantitative PCR Forward Primer
NR-9346
Orthopoxvirus Pan-Orthopox Virus E9L Gene-Specific Quantitative PCR Reverse Primer
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