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NR-9562   Yersinia pestis Plasmid Detection Kit
(Assays/Panels)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Yersinia pestis Plasmid Detection Kit
Organism: Yersinia pestis
Biosafety Level: 1
Availability Status: In Stock
Store at: -60°C or colder
Contributor: BEI Resources
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

The Yersinia pestis Plasmid Detection Kit is designed to detect the presence of pPCP1, pMT1 and pCD1. The kit consists of the following components:

  1. Primer sets designed to detect the pPCP1, pMT1 and pCD1 plasmids using standard polymerase chain reactions, resulting in amplicons of approximately 400, 1200 and 1900 base pairs, respectively
  2. Positive control primer set that amplifies an approximately 800 base pair chromosomal marker unique to Yersinia pestis
  3. Linearized plasmid internal control DNA; amplification using each of the primer sets will result in a band of approximately 130 base pairs
BEI Resources
Component
NR-9686
pPCP1 Primer Set
NR-9687
pMT1 Primer Set
NR-9688
pCD1 Primer Set
NR-9689
Yersinia pestis Primer Set
NR-9551
Internal DNA Control

BEI Resources NR-2715 (Genomic DNA from Yersinia pestis, strain ZE94-2122) contains all three plasmids and can be used as an additional positive control. The successful amplification of an approximately 1900 base pair band from this control DNA is essential when reporting results for the presence or absence of the pCD1 plasmid.  BEI Resources NR-2646 (Genomic DNA from Yersinia enterocolitica subsp. enterocolitica, strain 33114) contains none of the plasmids and may be used as a negative control.

NR-9686, NR-9687, NR-9688 and NR-9689 contain approximately 400 µL of a mixture of forward and reverse primers in TE buffer (10 mM Tris-HCl, 1 mM EDTA) (pH 7.0). NR-9551 contains approximately 40 ng of plasmid DNA in TE buffer (pH 7.0).
Components: NR-9551,NR-9686,NR-9687,NR-9688,NR-9689
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Yersinia pestis Plasmid Detection Kit, NR-9562."
PERMITS
For a list of permits that may be required for shipping this product and to set the permit information preferences; please select a country from the drop down below.

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

RELATED PRODUCTS
NR-2646
Genomic DNA from Yersinia enterocolitica subsp. enterocolitica, Strain 33114
NR-2715
Genomic DNA from Yersinia pestis, Strain ZE94-2122
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BEI Resources products are intended for laboratory research purposes only. They are not intended for use in humans.

While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this site, BEI Resources makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. BEI Resources does not warrant that such information has been confirmed to be accurate.


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