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Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
ARP-198 is a feline T cell line infected with the feline leukemia virus (FeLV), strain 61E. FeLV- induces persistent viremia and caused low incidence, long latency lymphoma in domestic cats. The plasmid clone is available as ARP-109.
Growth Characteristics: 3201B feline T-cells grow as a clumpy suspension with a doubling time of 15 to 20 hours. Maintain cultures at 0.5 × 106 to 3.0 × 106 cells per mL. Passage every 4 to 6 days. Please use the recommended growth medium, as the cells do not grow in other media.
Growth Media: RPMI 1640 Medium/Leibovitz's L-15 Medium (1:1) supplemented with 10% fetal bovine serum
ARP-198 is negative for aerobic and anaerobic bacteria, Mycoplasma, fungi and yeast.
Each vial of ARP-198 contains approximately 9.6 × 106 cells in RPMI 1640/Leibovit’s L-15 Media (1:1) supplemented with 50% fetal bovine serum and 10% dimethyl sulfoxide (DMSO). Please refer to the attached file(s) for additional information.
Snyder, H. W., et al. “Characterisation of a Tumour-Specific Antigen on the Surface of Feline Lymphosarcoma Cells.” Nature 275 (1978): 656-658. PubMed: 212681.
Overbaugh, J., et al. “Molecular Cloning of a Feline Leukemia Virus that Induces Fatal Immunodeficiency Disease in Cats.”Science 239 (1988): 906-910. PubMed: 2893454.
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