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ARP-234 is a CEMx174 cell line infected by phage clone pJSP4-27 of human immunodeficiency virus 2/ST (HIV-2/ST), which was originally isolated from the peripheral blood mononuclear cells of a healthy Senegalese prostitute. CEMx174 cells were generated by the fusion of human T cell line CEM and human B cell line 721.174.
ARP-234 expresses B cell, T cell, and class II human leukocyte antigen markers, and produces moderate to high level of virus as determined by reverse transcriptase assay. HIV-2/ST is less cytopathic than HIV-2/ROD.
Growth Characteristics: Cells grow as a suspension, with a doubling time of about 18 hours, and tend to grow in large clumps that are difficult to dissociate. Passage twice weekly, splitting to 1× 105 to 2 × 105 cells per mL. Maximum cell density is 1 × 106 to 2 × 106. Fresh cells can be added at a ratio of 1 infected to 2 uninfected.
Growth Media: RPMI 1640 supplemented with 15% fetal bovine serum, 2 mM L-glutamine, 100 U per mL penicillin and 100 µg per mL streptomycin
ARP-234 is negative for Mycoplasma, bacteria, mold and yeast.
Each vial of ARP-234 contains approximately 7.5 × 106 cells in RPMI 1640 medium supplemented with 50% fetal bovine serum and 10% dimethyl sulfoxide (DMSO). Please refer to the attached file(s) for additional information.
Kong, L. I., et al. “West African HIV-2-Related Human Retrovirus with Attenuated Cytopathicity.” Science 240 (1099): 1525-1529. PubMed: 3375832.
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