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-100°C or colder, preferably in the vapor phase of a liquid nitrogen freezer
ARP-312 is a human T-cell line, HUT 78, infected with Simian immunodeficiency virus (SIV-BK44).
Growth Characteristics: Cells grow in a clumpy suspension with a doubling time of 3 days. Cultures should be passaged cells every 3 to 4 days. At time of passage, feed the cells with 50% spent medium and 50% fresh medium. The addition of uninfected cells is not necessary. When thawing these cells, place vial immediately in a 37°C water bath. As soon as the cells have thawed, transfer them into a sterile 12 × 15 mm polypropylene centrifuge tube, and add fresh complete medium up to 10 mL. Gently resuspend cells, then centrifuge at 1200 rpm for 5 minutes. Aspirate off medium and resuspend cells in fresh, complete medium; centrifuge again for 5 minutes at 1200 rpm. Aspirate off medium, resuspend the cells in complete medium again, and transfer them to a T25 tissue culture flask.
Growth Medium: RPMI 1640 supplemented with 10% fetal bovine serum ARP-312 is negative for mycoplasma, bacteria and fungi. Each vial of ARP-312 contains approximately 6 x 106 cells in RPMI 1640 medium supplemented with 50% fetal bovine serum and 10% dimethyl sulfoxide (DMSO). Please refer to the attached file(s) for additional information.
Kornfeld, H., et al. “Cloning of HTLV-4 and Its Relation to Simian and Human Immunodeficiency Viruses.” Nature 326 (1987): 610-613. PubMed: 3104797.
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