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-100°C or colder, preferably in the vapor phase of a liquid nitrogen freezer
ARP-400 is the H9 cell line infected with human immunodeficiency virus 1 IIIB (HIV-1 IIIB). The H9 cell line was derived from the human T-cell line, HUT 78, as a single clone. HIV-1 IIIB has high capacity for replication in T cell lines and appears to be well adapted for in vitro culture in T cell lines.
Growth Characteristics: Cells grow as a single-cell suspension. Maintain cultures at 5 × 105 to 1 × 106 cells per mL for optimum growth and split every 2 to 3 days at a ratio of 1:2 to 1:4. When cells are first started in culture, split at 1:2 only for the first week. Cells will recover rapidly after 1 to 2 days of culture post-thawing.
Growth Medium: RPMI 1640 supplemented with 10% fetal bovine serum and 2 mM L-glutamine ARP-400 is negative for mycoplasma, bacteria and fungi. Each vial of ARP-400 contains approximately 4 x 106 cells in RPMI 1640 supplemented 40% fetal bovine serum and 10% dimethyl sulfoxide (DMSO). Please refer to the appropriate data sheet for lot-specific information.
Popovic, M., et al. “Detection, Isolation, and Continuous Production of Cytopathic Retroviruses (HTLV-III) from Patients with AIDS and Pre-AIDS.”Science 224 (1984): 497-500. PubMed: 6200935.
Popovic, M., E. Read-Connole and R. C. Gallo. “T4 Positive Human Neoplastic Cell Lines Susceptible to and Permissive for HTLV-III.”Lancet 2 (1984): 472-1473.PubMed: 6151082.
Ratner, L., et al. “Complete Nucleotide Sequence of the AIDS Virus, HTLV-III.” Nature 313 (1985): 277-284. PubMed: 2578615.
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